Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. The use of sodium phosphate buffer led to high recovery of a test protein (pI 9.2), while a Bis-Tris propane buffer (Good’s buffer) offered high impurity clearance. significant effects on the purity and recovery of the target protein during Nuvia aPrime 4A chromatography. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. In the present study, we show that buffer components have. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. XTractor Buffer has been optimized for extraction of tagged proteins, which can then be purified with any of our affinity resins, such as TALON Co Resin or His60 Ni Superflow Resin, or any other affinity resins. This method works especially well for high molecular weight proteins, such as beta-galactosidase ( LacZ), that cannot be extracted unless the membranes are completely disrupted. The buffer kit also includes lysozyme and DNase I. The xTractor Buffer Kit provides enough buffer to extract proteins from 10 g of bacterial pellet.
In addition, the biological activity of the protein obtained by xTractor Buffer extraction is higher than the activity of the protein obtained by sonication. When compared to sonication, xTractor Buffer extraction yielded more protein content from the same size sample. This cell lysis protocol is a highly efficient means of extracting proteins from any cells in order to obtain increased protein purity and yield. Composition/Information on Ingredients NA RI0175000R22-36-50/53 Hazardous Components (Chemical Name) Octylphenoxypolyethoxyethanol CAS 1.0 -5.0 Concentration EC Risk Phrases RTECS 1. To save time, the resulting lysate can be loaded directly on a TALON CellThru Column without centrifugation or filtration. Lysis Buffer SAFETY DATA SHEET Medical Conditions Generally Aggravated By No data available. After extraction, simply centrifuge or filter the lysate and load it on any affinity resin column to isolate your tagged proteins. The final lysate has no visible cell fragments or precipitates. Just resuspend the cell pellet in the cell lysis buffer (1:20 w/v) and mix gently for 10 minutes, reducing the risk of proteolysis. Spatial mapping of protein composition and tissue organization: a primer for multiplexed. xTractor Buffer has been optimized for extraction of his-tagged proteins, so it is compatible with all IMAC resins. xTractor Buffer is optimized for superior protein yield. This cell lysis reagent provides fast and efficient (10 min) lysis using mild, non-denaturing conditions. XTractor Buffer is the most flexible and efficient cell lysis buffer for protein extraction from bacterial, yeast, mammalian, and baculovirus-infected cells.
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